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Anshin'in and the other Not Equal, Hanten Shiranui, do not see themselves as above or below humanity, but rather see humanity as something that cannot begin to be compared with them, thus being "not equal" to them.

Anshin'in comes off as cold and calculated, always having the upper hand in any confrontation she appears in. This is due to her absurdly expansive arsenal of abilities. She holds 7,932,135,441,523,222 Abnormalities and 4,925,916,526,110,643 Minuses. This gives her 12,858,051,967,633,865 different skills to use. Her skills are classified into ten categories (Sword, Martial Arts, Magic, Mental, Biological, Boss, Gun, Ninja, Protagonist, and Miscellaneous) and include almost every conceivable power one can have, many with very open-ended limits.

One such example "Alibi Block", allows Anshin'in to appear wherever she wishes, whether that be to the next rooftop, behind a character, in someone's dreams, or even hell itself should she wish it.

Anshin'in is the chief architect of both the Test Tube Plan and the Flask Plan, which seek to create a perfect human, a seemingly impossible task. Three years before the series, while attending Hakobune Middle School to observe the first human she had expectations for, Misogi Kumagawa, she and Hanten Shiranui were sealed by him through the use of his minus, Book Maker, which strips people of their power and reduces them to Kumagawa's level. While sealed, she had little use of her more fantastical powers, but could still use simple ones such as Alibi Block. She frequently used this to appear in Kumagawa's dreams and mind in order to speak with him.

She was able to bargain with Kumagawa to give up his minus in exchange for a new one called "Hundred Gauntlets", which he later developed into "All Fiction". As Kumagawa began to change for the better due to his defeat at the hands of Kurokami Medaka, the seal which took the form of screws on her body began to vanish, and she transferred into Hakoniwa Academy to continue the Flask Plan.

Kurokami Medaka's Successor Arc Just as the student council learns of the threat that Anshin'in poses, she appears before them and explains that she seeks to restart the Flask Plan that they had just previously shut down. When faced with the prospect of having to fight Medaka, she declines, stating that if the world was a manga, then Medaka would be the main character, in which case Anshin'in would be fated to lose. As such, she simply decides to wait for Medaka to graduate so that she can fulfill her goal unimpeded.

The council begins its process to select successors to fight against Anshin'in to prevent her from succeeding, but it is revealed that all of the candidates, and approximately 700 million people in the world, have part of Anshin'in in them. As tensions grow in the student council, Zenkichi Hitoyoshi fights with Medaka and claims that he will defeat her. Seeing this as an impossible task, Anshin'in helps train Zenkichi along with the help of Hanten Shiranui, who creates the skill "Devil Style" for him, which nullifies any and all coincidence and plot convenience. In the student council election, Zenkichi is victorious over Medaka, something that Anshin'in considered impossible.

For something so impossible to be accomplished so perfectly, the world would have to be a manga. She had never found a problem she could not solve, and needed to confirm if there was anything which was impossible for her to accomplish. If something that seemed impossible really was, then that would confirm to Anshin'in that the life she was living was indeed real. The fact that the impossible was actually possible was proof that she lived in a manga, and this was something that Anshin'in could not accept, and attempted to take her own life.

However, she is stopped by Medaka, who considers her perception to be a "simulated reality", and is convinced to try living as a student for some time to get some perspective.

Jet Black Bride Arc As the second black wedding ceremony approaches, Medaka seeks out a team sufficiently powerful to defeat her suitors. She chooses Anshin'in along with Kamome Tsurubami, Hanten Shiranui, Hansode Shiranui, and Kumagawa. After arriving at the aircraft carrier, Black, Anshin'in swiftly defeats the doubles of the suitors using six hundred of her skills, allowing the group to move forward to the real suitors. Zenkichi claims that the weakness of the team he calls "All Jokers" is that they all do not dodge the first attack. This is utilized by style user Momo Momozono, who traps everyone except Medaka in cards.

Anshin'in idles about for some time, but then decides to break out after enjoying some of the seal a bit more. Medaka bargains for the release of Anshin'in and Hanten, and Anshin'in readies to defeat the rest of the suitors, but is stopped by Medaka so that she can play their game.

Unknown Shiranui Arc Anshin'in, along with Medaka, Kumagawa, and Zenkichi, travel to the Shiranui village to search out Hansode Shiranui after she asks Kumagawa to erase her enrollment at Hakoniwa Academy. Whilst there, the group is ambushed by Iihiko Shishime, Anshin'in's enemy of over five thousand years previously whom she could not defeat after over 100 million attempts. Anshin'in tells the others to escape while she holds Iihiko off, where she fights to the death for the first time. She is cut in half by a Iihiko, and presumed dead by her double, Hanten.

She meets with Kumagawa in his mind once more in what she claims is an automated message should she die and returns Kumagawa's Hundred Gauntlets which allowed him to use All Fiction again, as well as a new skill known as Unskilled. As Zenkichi prepares to have a final showdown with Iihiko, Anshin'in is one of the voices to call out to him, and then speaks directly to him to encourage him.

Anshin'in's message for Kurokami Medaka for the 100 flowers run is a request to play again, affirming in Medaka's and Zenkichi's minds that she is still alive, as she would not make a promise she could not keep.